Antibiotic resistance gene dissipation in soil microcosms amended with antibiotics and swine manure

The use of antibiotics in animal agriculture has exacerbated the presence both antibiotic resistance genes (ARGs) and residual excreted manure. Field application this manure is a common practice because its nutrient rich material can benefit crop growth. However, also introduce ARGs into nonagricultural settings. integration prairie buffer strips within at edge fields potential management solution to reduce concentrations commonly transported via water runoff infiltration. An incubation experiment was conducted investigate fate directly manured field soils surrounding affected strip soils. Row sampled from three sites received either an spike swine addition or control addition. select were then monitored over 72-d period. Although soil vegetation site location not observed influence ARG dissipation, did display different half-lives one another. For example, tetM demonstrated fastest dissipation quantified (average half-life, 5.18 d). Conversely, sul1 conform first-order linear regression kinetics used describe other investigated highly abundant these are comparable previous studies inform monitoring mitigative efforts aimed reducing spread environment.